Isolation, Purification, and Authentication of Rhizobial Isolates from Legume Nodules
Complete laboratory protocol for rhizobia isolation including plant trap method, nodule collection at flowering, surface sterilization procedures (ethanol/sodium hypochlorite), YEMA medium culturing, purification via streak plate method, confirmatory tests (Congo Red absorption, Peptone-Glucose), cultural characteristics assessment, and physiological tolerance testing (temperature, salt, pH, phosphorus solubilization).