Complete methodology for isolating free-living nitrogen-fixing Azotobacter (oval/spherical, motile, gram-negative) and phosphate-solubilizing Phosphobacteria (Bacillus megaterium) from soil samples via serial dilution. Includes biochemical tests (citrate, catalase, urease), growth on selective media (Ashby’s agar, Pikovskaya’s medium), mass production using bioreactors (fermentation 4-9 days, 10⁸-10⁹ cells/mL), carrier formulation, and biofertilizer preparation.
