Microbial Culture Preservation - Methods and Long-Term Storage Technique
Summary :Comprehensive guide covering culture preservation methods: serial subculture (routine, cost-effective), mineral oil overlay (15-20 year viability), lyophilization/freeze-drying (indefinite storage at -130°C), liquid nitrogen storage (-196°C, cryogenic), soil cultures, silica gel storage, and water storage. Includes advantages/disadvantages, hazard prevention, documentation standards, and institutional culture collection maintenance systems.